Hotspot

petA-psbJ

LSC · 61794-62572 · 779 bp

Back to Gymnadenia

Pi 0.00924

MarkerSeek score 37.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 779
variable_sites: 13
pi: 0.00924
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTGATTTTGGCAC	TATTCCGGGTTGGGTTCATC	50.0	50.0	58.0	57.3	891-903 bp	1.0	75.4
petA-psbJ_p2	GGCATCTGTGATTTTGGCAC	CTATTCCGGGTTGGGTTCAT	50.0	50.0	58.0	57.3	892-904 bp	1.0	75.3
petA-psbJ_p3	GAGTCCAAGGCCTTCTGTTC	TATTCCGGGTTGGGTTCATC	55.0	50.0	58.2	57.3	916-928 bp	1.0	73.9
petA-psbJ_p4	GAGTCCAAGGCCTTCTGTTC	CTATTCCGGGTTGGGTTCAT	55.0	50.0	58.2	57.3	917-929 bp	1.0	73.8
petA-psbJ_p5	GTCCAAGGCCTTCTGTTCTT	TATTCCGGGTTGGGTTCATC	50.0	50.0	57.7	57.3	914-926 bp	1.0	73.1