Hotspot

atpB-rbcL

LSC · 53202-53964 · 763 bp

Back to Gymnadenia

Pi 0.00367

MarkerSeek score 54.00

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 763
variable_sites: 6
pi: 0.00367
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGAGTGATACGCCCTAGGTT	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.8	58.1	871-876 bp	1.0	78.5
atpB-rbcL_p2	TTGAGTGATACGCCCTAGGT	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.8	58.1	872-877 bp	1.0	78.5
atpB-rbcL_p3	TGAGTGATACGCCCTAGGTT	TGGTGACATAAGTCCCTCCC	50.0	55.0	57.8	58.4	872-877 bp	1.0	76.0
atpB-rbcL_p4	TTGAGTGATACGCCCTAGGT	TGGTGACATAAGTCCCTCCC	50.0	55.0	57.8	58.4	873-878 bp	1.0	76.0
atpB-rbcL_p5	TGAGTGATACGCCCTAGGTT	TCCAACGCTTGCTTTAGTCT	50.0	45.0	57.8	57.5	899-904 bp	1.0	75.0