Hotspot

ycf3-trnS-GGA

LSC · 43454-44044 · 591 bp

Back to Gymnadenia

Pi 0.00541

MarkerSeek score 53.90

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 591
variable_sites: 7
pi: 0.00541
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTGAA	45.0	45.0	56.4	57.8	725-730 bp	1.0	66.6
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	TTCCAATGCTACGCCTTGAA	45.0	45.0	56.4	57.8	726-731 bp	1.0	66.6
ycf3-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	CGGAGAGAGAGGGATTCGAA	45.0	55.0	56.4	58.3	774-779 bp	1.0	65.5
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	CGGAGAGAGAGGGATTCGAA	45.0	55.0	56.4	58.3	775-780 bp	1.0	65.5
ycf3-trnS-GGA_p5	TCTCCTGAAGTTGTCGGAAT	TTCCAATGCTACGCCTTGAA	45.0	45.0	56.2	57.8	724-729 bp	1.0	64.7