Hotspot

trnE-UUC-trnT-GGU

LSC · 30171-31032 · 862 bp

Back to Gymnadenia

Pi 0.00186

MarkerSeek score 54.00

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 862
variable_sites: 4
pi: 0.00186
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.8	58.1	937-947 bp	1.0	79.3
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.8	58.1	934-944 bp	1.0	79.1
trnE-UUC-trnT-GGU_p3	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.7	58.1	915-925 bp	1.0	78.5
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.7	58.1	912-922 bp	1.0	78.3
trnE-UUC-trnT-GGU_p5	CGTTGCCTCCTTGAAAGAGA	AACTTACGCCTTACCATGGC	50.0	50.0	57.8	58.3	950-960 bp	1.0	77.8