Hotspot

atpH-atpI

LSC · 12916-13477 · 562 bp

Back to Gymnadenia

Pi 0.00605

MarkerSeek score 58.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 562
variable_sites: 8
pi: 0.00605
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCAGCGGAAAT	TTTTTGCAACTTTGGCTGCA	45.0	40.0	58.0	57.9	640-652 bp	1.0	82.2
atpH-atpI_p2	AGCCAATCCAGCAGCAATAA	TTTTTGCAACTTTGGCTGCA	45.0	40.0	57.9	57.9	656-668 bp	1.0	81.2
atpH-atpI_p3	AATAACGGAAGCAGCGGAAA	TTTTTGCAACTTTGGCTGCA	45.0	40.0	58.2	57.9	641-653 bp	1.0	81.0
atpH-atpI_p4	GCCAATCCAGCAGCAATAAC	TTTTTGCAACTTTGGCTGCA	50.0	40.0	57.8	57.9	655-667 bp	1.0	80.7
atpH-atpI_p5	TCCAGCAGCAATAACGGAAG	TTTTTGCAACTTTGGCTGCA	50.0	40.0	58.3	57.9	650-662 bp	1.0	80.4