Hotspot

trnK-UUU-rps16

LSC · 4252-4894 · 643 bp

Back to Gymnadenia

Pi 0.00871

MarkerSeek score 53.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 643
variable_sites: 12
pi: 0.00871
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	GCAAAGACAACGAATTCCGG	50.0	50.0	58.2	58.0	1279-1291 bp	1.0	81.1
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	GCAAAGACAACGAATTCCGG	50.0	50.0	58.2	58.0	1278-1290 bp	1.0	81.1
trnK-UUU-rps16_p3	CAGGATCAGTCGCGGTATTC	GCAAAGACAACGAATTCCGG	55.0	50.0	58.2	58.0	1365-1377 bp	1.0	80.5
trnK-UUU-rps16_p4	AAAGCCGAGTACTCTACCGT	TCGATCCCGAAGAGAAGGAA	50.0	50.0	58.2	57.9	853-860 bp	1.0	79.7
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	TCGATCCCGAAGAGAAGGAA	50.0	50.0	58.2	57.9	852-859 bp	1.0	79.7