Hotspot

matK-trnK-UUU

LSC · 3173-4214 · 1042 bp

Back to Gymnadenia

Pi 0.00788

MarkerSeek score 54.20

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 1042
variable_sites: 17
pi: 0.00788
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TCCTGAAGGAGTAGCGGATA	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.0	58.2	1142-1162 bp	1.0	72.8
matK-trnK-UUU_p2	TCCTGAAGGAGTAGCGGATA	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.0	58.2	1141-1161 bp	1.0	72.8
matK-trnK-UUU_p3	TCCTGAAGGAGTAGCGGATA	GGGTTGCTAACTCAACGGTA	50.0	50.0	57.0	57.5	1155-1175 bp	1.0	70.4
matK-trnK-UUU_p4	TCCTGAAGGAGTAGCGGATAT	AACGGTAGAGTACTCGGCTT	47.6	50.0	57.5	58.2	1142-1162 bp	1.0	68.9
matK-trnK-UUU_p5	TCCTGAAGGAGTAGCGGATAT	ACGGTAGAGTACTCGGCTTT	47.6	50.0	57.5	58.2	1141-1161 bp	1.0	68.9