Hotspot

ycf1

SSC · 10775-16375 · 5601 bp

Back to Guzmania

Pi 0.00858

MarkerSeek score 46.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5601
variable_sites: 48
pi: 0.00858
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGAAATAGATCCTGTGAATGGT	TCAACCATTCAAATCCAATCCA	39.1	36.4	57.3	56.2	305 bp	1.0	41.3
ycf1_p2	TGGAAATAGATCCTGTGAATGGT	ACCATTCAAATCCAATCCATGA	39.1	36.4	57.3	56.0	302 bp	1.0	41.3
ycf1_p3	TGGAAATAGATCCTGTGAATGGT	CAACCATTCAAATCCAATCCATGA	39.1	37.5	57.3	58.0	304 bp	1.0	41.3
ycf1_p4	TGGAAATAGATCCTGTGAATGGT	TCAACCATTCAAATCCAATCCATG	39.1	37.5	57.3	58.0	305 bp	1.0	41.3
ycf1_p5	TTGGAAATAGATCCTGTGAATGGT	TCAACCATTCAAATCCAATCCA	37.5	36.4	57.9	56.2	306 bp	1.0	41.3