Hotspot

ndhC-trnV-UAC

LSC · 93277-94322 · 1046 bp

Back to Guzmania

Pi 0.0205

MarkerSeek score 72.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1046
variable_sites: 21
pi: 0.0205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTAACCGGGGCTAAAACTCC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.0	57.8	1183-1212 bp	1.0	81.0
ndhC-trnV-UAC_p2	CTAACCGGGGCTAAAACTCC	GAGCCCAATGCAACAAACAA	55.0	45.0	58.0	57.8	1270-1299 bp	1.0	80.5
ndhC-trnV-UAC_p3	CGGGGCTAAAACTCCTGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1178-1207 bp	1.0	79.8
ndhC-trnV-UAC_p4	CGGGGCTAAAACTCCTGAAA	GAGCCCAATGCAACAAACAA	50.0	45.0	57.8	57.8	1265-1294 bp	1.0	79.2
ndhC-trnV-UAC_p5	TAACCGGGGCTAAAACTCCT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.3	57.8	1182-1211 bp	1.0	78.6