Hotspot

psbM-trnD-GUC

LSC · 72578-73611 · 1034 bp

Back to Guzmania

Pi 0.0145

MarkerSeek score 63.60

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1034
variable_sites: 15
pi: 0.0145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	CCTCGCTGGGATTGTAGTTC	45.0	55.0	55.8	58.1	1162-1164 bp	1.0	62.7
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	TACACCTCGCTGGGATTGTA	45.0	50.0	55.8	58.1	1166-1168 bp	1.0	62.0
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	CATACACCTCGCTGGGATTG	45.0	55.0	55.8	58.4	1168-1170 bp	1.0	59.9
psbM-trnD-GUC_p4	AGGAACTAGAATGAACAGTGCA	CCTCGCTGGGATTGTAGTTC	40.9	55.0	57.4	58.1	1169-1171 bp	1.0	59.6
psbM-trnD-GUC_p5	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	1145-1147 bp	1.0	59.4