Hotspot

atpH-atpI

LSC · 55447-56426 · 980 bp

Back to Guzmania

Pi 0.00826

MarkerSeek score 64.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 980
variable_sites: 8
pi: 0.00826
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	1037-1045 bp	1.0	79.7
atpH-atpI_p2	CAGTACCTTGACCAACTCCG	CGAATCCATGGAGGGTCATC	55.0	55.0	57.9	57.8	1096-1104 bp	1.0	79.3
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	CGAATCCATGGAGGGTCATC	45.0	55.0	57.9	57.8	1053-1061 bp	1.0	78.8
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.2	57.8	1038-1046 bp	1.0	78.6
atpH-atpI_p5	GAAGCAAGTCCTACAGCCAA	CGAATCCATGGAGGGTCATC	50.0	55.0	57.8	57.8	1067-1075 bp	1.0	78.5