Hotspot

rpl32-trnL-UAG

SSC · 175-976 · 802 bp

Back to Guzmania

Pi 0.0227

MarkerSeek score 72.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 802
variable_sites: 18
pi: 0.0227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CACCGGGCATTCAAAAAGTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.5	58.0	883-889 bp	1.0	78.1
rpl32-trnL-UAG_p2	CACCGGGCATTCAAAAAGTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.5	58.0	884-890 bp	1.0	78.1
rpl32-trnL-UAG_p3	CACCGGGCATTCAAAAAGTT	TTTAGAAGATGCCGCCACTC	45.0	50.0	57.5	58.0	943-949 bp	1.0	77.8
rpl32-trnL-UAG_p4	TTCCACCGGGCATTCAAAAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.6	58.0	886-892 bp	1.0	77.6
rpl32-trnL-UAG_p5	TTTCCACCGGGCATTCAAAA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.6	58.0	887-893 bp	1.0	77.6