Hotspot

rnl

IRb · 50934-53800 · 2867 bp

Back to Guinardia

Pi 0.485
MarkerSeek score 77.80
Primer available yes
Label rnl

Feature Metadata

label_name
rnl
feature_type
rRNA
region
IRb
length_bp
2867
variable_sites
1177
pi
0.485
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.85
markerseek_score
77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rnl_p1 ACCTCCACAAGTAATAACAAA AGCCTAAAATATTAACGATACAATTGT 33.3 25.9 52.7 56.0 3000 bp 0.5 20.0
rnl_p2 ACCTCCACAAGTAATAACAAAA AGCCTAAAATATTAACGATACAATTGT 31.8 25.9 53.6 56.0 3000 bp 0.5 20.0
rnl_p3 ACCTCCACAAGTAATAACAAA GCCTAAAATATTAACGATACAATTGT 33.3 26.9 52.7 54.8 2999 bp 0.5 20.0
rnl_p4 ACCTCCACAAGTAATAACAAAA GCCTAAAATATTAACGATACAATTGT 31.8 26.9 53.6 54.8 2999 bp 0.5 20.0
rnl_p5 ACCTCCACAAGTAATAACAAA AGCCTAAAATATTAACGATACAATTG 33.3 26.9 52.7 54.6 3000 bp 0.5 20.0