Hotspot

psbA

IRb · 54471-55553 · 1083 bp

Back to Guinardia

Pi 0.498

MarkerSeek score 81.80

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: IRb
length_bp: 1083
variable_sites: 478
pi: 0.498
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	CGCTGATCCATACCGAACTC	TCGGACAAGCAGTTTCACAA	55.0	45.0	58.2	58.0	2001 bp	0.5	57.9
psbA_p2	CGCTGATCCATACCGAACTC	GTTTCACAACGCTTACAGCC	55.0	50.0	58.2	58.0	1990 bp	0.5	57.9
psbA_p3	CGCTGATCCATACCGAACTC	ACACGTGGTGAAGAAGCAAT	55.0	45.0	58.2	58.0	1957 bp	0.5	57.9
psbA_p4	CGCTGATCCATACCGAACTC	TTGGAATTATCGCAGGTGGG	55.0	50.0	58.2	57.9	2363 bp	0.5	57.8
psbA_p5	CGCTGATCCATACCGAACTC	GAATTATCGCAGGTGGGGTT	55.0	50.0	58.2	57.9	2360 bp	0.5	57.8