Hotspot

trnP(tgg)-acpP

IRa · 102534-103028 · 495 bp

Back to Guinardia

Pi 0.235

MarkerSeek score 81.20

Primer available yes

Label trnP(tgg)-acpP

Feature Metadata

label_name: trnP(tgg)-acpP
feature_type: igs
region: IRa
length_bp: 495
variable_sites: 111
pi: 0.235
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP(tgg)-acpP_p1	AGCTGCTCGCATTTGACATA	ATTAATGATGAGGCGGCAGG	45.0	50.0	58.0	58.1	1071 bp	0.5	59.0
trnP(tgg)-acpP_p2	AGCTGCTCGCATTTGACATA	AGCTTCGTCCATCTACACCT	45.0	50.0	58.0	58.1	1279 bp	0.5	58.6
trnP(tgg)-acpP_p3	AAAACTCCGGTAAATGCGGT	ATTAATGATGAGGCGGCAGG	45.0	50.0	58.1	58.1	1166 bp	0.5	58.4
trnP(tgg)-acpP_p4	AAACTCCGGTAAATGCGGTT	ATTAATGATGAGGCGGCAGG	45.0	50.0	58.1	58.1	1165 bp	0.5	58.4
trnP(tgg)-acpP_p5	AGCTGCTCGCATTTGACATA	GAGGCAGACCAAGTTACACC	45.0	55.0	58.0	58.2	1179 bp	0.5	58.3