Hotspot

trnI(gat)-rns

IRa · 99719-99782 · 64 bp

Back to Guinardia

Pi 0.105
MarkerSeek score 81.80
Primer available yes
Label trnI(gat)-rns

Feature Metadata

label_name
trnI(gat)-rns
feature_type
igs
region
IRa
length_bp
64
variable_sites
6
pi
0.105
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI(gat)-rns_p1 ATTTGAACCAGAGACCTCGC GACTGGGGTGAAGTCGTAAC 50.0 55.0 57.9 57.9 181-186 bp 1.0 84.3
trnI(gat)-rns_p2 CTGGGCCATTCTGGATTTGA GACTGGGGTGAAGTCGTAAC 50.0 55.0 57.9 57.9 195-200 bp 1.0 83.5
trnI(gat)-rns_p3 ATTTGAACCAGAGACCTCGC GGGGTGAAGTCGTAACAAGG 50.0 55.0 57.9 58.2 177-182 bp 1.0 83.4
trnI(gat)-rns_p4 AGAGACCTCGCCCTTATCAG GACTGGGGTGAAGTCGTAAC 55.0 55.0 58.3 57.9 172-177 bp 1.0 82.9
trnI(gat)-rns_p5 CTGGGCCATTCTGGATTTGA GGGGTGAAGTCGTAACAAGG 50.0 55.0 57.9 58.2 191-196 bp 1.0 82.6