Hotspot

psbA-rrn5

IRa · 95970-96454 · 485 bp

Back to Guinardia

Pi 0.402

MarkerSeek score 84.40

Primer available yes

Label psbA-rrn5

Feature Metadata

label_name: psbA-rrn5
feature_type: igs
region: IRa
length_bp: 485
variable_sites: 191
pi: 0.402
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-rrn5_p1	AACGCTCACAACTTCCCATT	GGGACCTCCTGGGAAGATAG	45.0	60.0	58.0	58.3	599-679 bp	1.0	86.9
psbA-rrn5_p2	GTAACGCTCACAACTTCCCA	GGGACCTCCTGGGAAGATAG	50.0	60.0	58.1	58.3	601-681 bp	1.0	86.0
psbA-rrn5_p3	TGGAAGTAATGCACGAACGT	GGGACCTCCTGGGAAGATAG	45.0	60.0	57.8	58.3	619-699 bp	1.0	85.7
psbA-rrn5_p4	AACGCTCACAACTTCCCATT	GGACCTCCTGGGAAGATAGT	45.0	55.0	58.0	57.5	598-678 bp	1.0	85.3
psbA-rrn5_p5	GTAATGCACGAACGTAACGC	GGGACCTCCTGGGAAGATAG	50.0	60.0	58.2	58.3	614-694 bp	1.0	85.2