Hotspot

trnS(tga)-petB

LSC · 32847-32934 · 88 bp

Back to Guinardia

Pi 0.277

MarkerSeek score 81.10

Primer available yes

Label trnS(tga)-petB

Feature Metadata

label_name: trnS(tga)-petB
feature_type: igs
region: LSC
length_bp: 88
variable_sites: 23
pi: 0.277
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS(tga)-petB_p1	GATGGCAGAGTTGGTCGATT	TCGTTCTTCAAACCAATCGT	50.0	40.0	58.0	55.6	202-204 bp	1.0	70.8
trnS(tga)-petB_p2	TGGTCGATTGCGTCTGATTT	TCGTTCTTCAAACCAATCGT	45.0	40.0	57.9	55.6	191-193 bp	1.0	70.4
trnS(tga)-petB_p3	TTGGTCGATTGCGTCTGATT	TCGTTCTTCAAACCAATCGT	45.0	40.0	57.9	55.6	192-194 bp	1.0	70.3
trnS(tga)-petB_p4	ATGGCAGAGTTGGTCGATTG	TCGTTCTTCAAACCAATCGT	50.0	40.0	58.3	55.6	201-203 bp	1.0	68.9
trnS(tga)-petB_p5	GGTCGATTGCGTCTGATTTG	TCGTTCTTCAAACCAATCGT	50.0	40.0	57.6	55.6	190-192 bp	1.0	67.6