Hotspot

ycf1

SSC · 112156-117573 · 5418 bp

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Pi 0.18

MarkerSeek score 76.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5418
variable_sites: 903
pi: 0.18
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATCGTTCCATAGCGAATCGG	TCAAGATTTACTCGGCCTGC	50.0	50.0	57.9	58.0	247 bp	0.5	59.3
ycf1_p2	ATCGTTCCATAGCGAATCGG	TTTACTCGGCCTGCGTATTC	50.0	50.0	57.9	58.1	241 bp	0.5	58.9
ycf1_p3	TGGATTTTGGGGCTTGGATC	TCAAGATTTACTCGGCCTGC	50.0	50.0	58.1	58.0	364 bp	0.5	58.7
ycf1_p4	AGATCGCCATTCCAAGCAAA	TCAAGATTTACTCGGCCTGC	45.0	50.0	58.2	58.0	560 bp	0.5	58.5
ycf1_p5	TGGATTTTGGGGCTTGGATC	TTTACTCGGCCTGCGTATTC	50.0	50.0	58.1	58.1	358 bp	0.5	58.4