Hotspot

ndhA

SSC · 119315-121641 · 2327 bp

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Pi 0.47

MarkerSeek score 80.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2327
variable_sites: 965
pi: 0.47
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GTGAGAGTTGAAGCCCCAAA	CGATTTGCGTGGGATAAGGT	50.0	50.0	58.0	58.3	2712 bp	0.5	57.2
ndhA_p2	CAGGCTCTGGAAGGAATTCC	CGATTTGCGTGGGATAAGGT	55.0	50.0	58.0	58.3	2865 bp	0.5	56.9
ndhA_p3	GTGAGAGTTGAAGCCCCAAA	TTGACCAATGTACCTTGCGG	50.0	50.0	58.0	58.5	2680 bp	0.5	56.1
ndhA_p4	CAGGCTCTGGAAGGAATTCC	TTGACCAATGTACCTTGCGG	55.0	50.0	58.0	58.5	2833 bp	0.5	55.8
ndhA_p5	GTGAGAGTTGAAGCCCCAAA	CGGCTCGTACTGTTTGTTGA	50.0	50.0	58.0	58.5	2663 bp	0.5	55.8