Hotspot

trnL-UAG-rpl32

SSC · 127226-127598 · 373 bp

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Pi 0.46

MarkerSeek score 77.80

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 373
variable_sites: 165
pi: 0.46
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	AGCGTGTCTACCGATTTCAC	GGGATATTGGGCAGCATTGA	50.0	50.0	58.0	58.0	502 bp	0.5	59.9
trnL-UAG-rpl32_p2	CAGCGTGTCTACCGATTTCA	GGGATATTGGGCAGCATTGA	50.0	50.0	58.0	58.0	503 bp	0.5	59.9
trnL-UAG-rpl32_p3	AGCGTGTCTACCGATTTCAC	AGGGATATTGGGCAGCATTG	50.0	50.0	58.0	58.0	503 bp	0.5	59.9
trnL-UAG-rpl32_p4	CAGCGTGTCTACCGATTTCA	AGGGATATTGGGCAGCATTG	50.0	50.0	58.0	58.0	504 bp	0.5	59.9
trnL-UAG-rpl32_p5	GCTGCATAGCCCAATACCAT	GGGATATTGGGCAGCATTGA	50.0	50.0	58.1	58.0	1108 bp	0.5	59.3