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psbZ-trnG-GCC

LSC · 38737-39259 · 523 bp

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Pi 0.0373

MarkerSeek score 80.90

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 523
variable_sites: 19
pi: 0.0373
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	GCGTCTTCTCTTTGGCAAAG	55.0	50.0	58.0	57.7	678-705 bp	1.0	81.0
psbZ-trnG-GCC_p2	TTGCTTCTCCTGATGGTTGG	GCGTCTTCTCTTTGGCAAAG	50.0	50.0	57.8	57.7	681-708 bp	1.0	79.5
psbZ-trnG-GCC_p3	TCCTGATGGTTGGTCGAGTA	GCGTCTTCTCTTTGGCAAAG	50.0	50.0	57.8	57.7	674-701 bp	1.0	79.3
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTC	GCGTCTTCTCTTTGGCAAAG	55.0	50.0	58.3	57.7	679-706 bp	1.0	79.1
psbZ-trnG-GCC_p5	TTCTCCTGATGGTTGGTCGA	GCGTCTTCTCTTTGGCAAAG	50.0	50.0	58.4	57.7	677-704 bp	1.0	78.2