Hotspot

trnR-UCU-atpA

LSC · 10466-10755 · 290 bp

Back to Guilandina

Pi 0.0621

MarkerSeek score 82.80

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 290
variable_sites: 18
pi: 0.0621
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	AAGACATTCACTCGGGAAGC	47.6	50.0	57.3	57.9	408-493 bp	1.0	68.8
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	CCAAGACATTCACTCGGGAA	47.6	50.0	57.3	57.5	410-495 bp	1.0	65.9
trnR-UCU-atpA_p3	TGGATAGGACAGAGGTCTTCT	AAGACATTCACTCGGGAAGC	47.6	50.0	56.9	57.9	443-528 bp	1.0	65.7
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	ACCAAGACATTCACTCGGGA	47.6	50.0	57.3	58.7	411-496 bp	1.0	64.4
trnR-UCU-atpA_p5	GTTCAAATCCTATTGGACGCA	AAGACATTCACTCGGGAAGC	42.9	50.0	56.6	57.9	407-492 bp	1.0	63.2