Hotspot

rps16-trnQ-UUG

LSC · 6340-6942 · 603 bp

Back to Guilandina

Pi 0.133
MarkerSeek score 89.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
603
variable_sites
75
pi
0.133
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
89.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GTCTTTCAAGTCGCACGTTG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 683-812 bp 1.0 91.3
rps16-trnQ-UUG_p2 ATGTCTTTCAAGTCGCACGT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 685-814 bp 1.0 90.3
rps16-trnQ-UUG_p3 GCACGTTGCTTTTTACCACA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 671-800 bp 1.0 89.8
rps16-trnQ-UUG_p4 CATGTCTTTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.8 58.1 686-815 bp 1.0 89.6
rps16-trnQ-UUG_p5 TGTCTTTCAAGTCGCACGTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.4 58.1 684-813 bp 1.0 88.6