Hotspot

rps15-ycf1

SSC · 128917-129314 · 398 bp

Back to Guibourtia

Pi 0.0255

MarkerSeek score 71.00

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 398
variable_sites: 20
pi: 0.0255
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TTGAAATTCAACCGATCCCG	GCAGCCGTTTCAGTATGGTA	45.0	50.0	56.4	58.0	523-555 bp	1.0	74.2
rps15-ycf1_p2	TTGAAATTCAACCGATCCCG	AATGGCAGCCGTTTCAGTAT	45.0	45.0	56.4	57.9	527-559 bp	1.0	73.3
rps15-ycf1_p3	TTGAAATTCAACCGATCCCG	ATAATGGCAGCCGTTTCAGT	45.0	45.0	56.4	57.9	529-561 bp	1.0	73.3
rps15-ycf1_p4	TTGAAATTCAACCGATCCCG	CCAATAATGGCAGCCGTTTC	45.0	50.0	56.4	57.8	532-564 bp	1.0	72.6
rps15-ycf1_p5	TTTGAAATTCAACCGATCCCG	GCAGCCGTTTCAGTATGGTA	42.9	50.0	57.2	58.0	524-556 bp	1.0	72.1