Hotspot

rpl32-trnL-UAG

SSC · 118194-119380 · 1187 bp

Back to Guibourtia

Pi 0.0304

MarkerSeek score 68.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1187
variable_sites: 66
pi: 0.0304
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCCACATCTCTTTCTACGGG	AGCGTGTCTACCGATTTCAC	55.0	50.0	58.1	58.0	1225-1330 bp	1.0	87.7
rpl32-trnL-UAG_p2	GCCACATCTCTTTCTACGGG	CAGCGTGTCTACCGATTTCA	55.0	50.0	58.1	58.0	1226-1331 bp	1.0	87.7
rpl32-trnL-UAG_p3	ACGGGGAACTCAAAAAGCTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	1210-1315 bp	1.0	87.7
rpl32-trnL-UAG_p4	ACGGGGAACTCAAAAAGCTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	1211-1316 bp	1.0	87.7
rpl32-trnL-UAG_p5	GCCACATCTCTTTCTACGGG	GCTGCATAGCCCAATACCAT	55.0	50.0	58.1	58.1	1825-1925 bp	1.0	86.2