Hotspot

rps3-rps19

LSC · 88006-88787 · 782 bp

Back to Guibourtia

Pi 0.0612

MarkerSeek score 80.00

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 782
variable_sites: 81
pi: 0.0612
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	TACCAAGTCTGAAACCGAGC	TCTAAACTTTCGGGGACACG	50.0	50.0	57.5	57.6	780-892 bp	1.0	84.6
rps3-rps19_p2	GTCTGAAACCGAGCGGATTT	TCTAAACTTTCGGGGACACG	50.0	50.0	58.6	57.6	774-886 bp	1.0	83.8
rps3-rps19_p3	TCTGAAACCGAGCGGATTTA	TCTAAACTTTCGGGGACACG	45.0	50.0	57.0	57.6	773-885 bp	1.0	79.9
rps3-rps19_p4	TACCAAGTCTGAAACCGAGC	TAAACTTTCGGGGACACGC	50.0	52.6	57.5	58.1	778-890 bp	1.0	79.5
rps3-rps19_p5	TACCAAGTCTGAAACCGAGC	GGGGACACGCCAAAAATGAT	50.0	50.0	57.5	59.1	769-881 bp	1.0	79.4