Hotspot

trnF-GAA-ndhJ

LSC · 52199-52984 · 786 bp

Back to Guibourtia

Pi 0.0222

MarkerSeek score 70.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 786
variable_sites: 33
pi: 0.0222
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATGCCCGAAAGTTGGATAGG	55.0	50.0	58.0	57.7	731-937 bp	1.0	84.3
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATGCCCGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	695-901 bp	1.0	84.0
trnF-GAA-ndhJ_p3	AGAATGGTCGGGATAGCTCA	ATGCCCGAAAGTTGGATAGG	50.0	50.0	57.9	57.7	740-946 bp	1.0	83.6
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	ATGCCCGAAAGTTGGATAGG	55.0	50.0	57.9	57.7	719-925 bp	1.0	83.3
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	GCCCGAAAGTTGGATAGGAT	55.0	50.0	58.0	57.4	729-935 bp	1.0	82.0