Hotspot

trnT-UGU-trnL-UAA

LSC · 50176-50992 · 817 bp

Back to Guibourtia

Pi 0.0553

MarkerSeek score 83.10

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 817
variable_sites: 77
pi: 0.0553
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.9	58.1	668-970 bp	1.0	89.6
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	674-976 bp	1.0	88.2
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	TACCAATTTCGCCATACCCC	55.0	50.0	57.9	57.6	661-963 bp	1.0	87.4
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	TAAGTCCGTAGCGTCTACCA	55.0	50.0	57.9	57.6	676-978 bp	1.0	86.9
trnT-UGU-trnL-UAA_p5	CTAACCTCTGAGCTAAGCGG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.5	58.1	660-962 bp	1.0	86.7