Hotspot

rps4-trnT-UGU

LSC · 49624-50102 · 479 bp

Back to Guibourtia

Pi 0.037
MarkerSeek score 69.00
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
479
variable_sites
31
pi
0.037
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.94
markerseek_score
69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 TAAAGACCCCAGACGACGTA GGTTCGATTCCGATAGCCG 50.0 57.9 57.8 58.2 414-611 bp 1.0 77.7
rps4-trnT-UGU_p2 GAAACGAGGCCCTCTGTAAC GGTTCGATTCCGATAGCCG 55.0 57.9 58.3 58.2 387-584 bp 1.0 77.3
rps4-trnT-UGU_p3 TTTTTGAAACGAGGCCCTCT GGTTCGATTCCGATAGCCG 45.0 57.9 57.6 58.2 392-589 bp 1.0 76.6
rps4-trnT-UGU_p4 TTTTGAAACGAGGCCCTCTG GGTTCGATTCCGATAGCCG 50.0 57.9 58.4 58.2 391-588 bp 1.0 76.4
rps4-trnT-UGU_p5 GACCCCAGACGACGTATTTT GGTTCGATTCCGATAGCCG 50.0 57.9 57.6 58.2 410-607 bp 1.0 76.3