Hotspot

psbZ-trnG-UCC

LSC · 39375-40090 · 716 bp

Back to Guibourtia

Pi 0.0708

MarkerSeek score 78.70

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 716
variable_sites: 82
pi: 0.0708
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	CTTCTCCTGATGGTTGGTCG	CGATCATAGGAGGGTCCCTT	55.0	55.0	58.0	58.0	980-1449 bp	1.0	91.7
psbZ-trnG-UCC_p2	CTTCTCCTGATGGTTGGTCG	CTTGAGGTCACGGGTTCAAA	55.0	50.0	58.0	58.0	822-1291 bp	1.0	91.6
psbZ-trnG-UCC_p3	CTTCTCCTGATGGTTGGTCG	CGACGTTGTGTTTTAACCGG	55.0	50.0	58.0	58.0	1147-1616 bp	1.0	90.9
psbZ-trnG-UCC_p4	CAACCACTCAGCCATCTCTC	CGATCATAGGAGGGTCCCTT	55.0	55.0	58.0	58.0	1455-1937 bp	1.0	90.1
psbZ-trnG-UCC_p5	CAACCACTCAGCCATCTCTC	CGACGTTGTGTTTTAACCGG	55.0	50.0	58.0	58.0	1622-2104 bp	1.0	89.7