Hotspot

atpI-rps2

LSC · 16879-17161 · 283 bp

Back to Guibourtia

Pi 0.023

MarkerSeek score 68.50

Primer available yes

Label atpI-rps2

Feature Metadata

label_name: atpI-rps2
feature_type: igs
region: LSC
length_bp: 283
variable_sites: 12
pi: 0.023
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-rps2_p1	CTTGGGCATGGACTTGGAAA	TGCAATTTGTGAGGGTCGTT	50.0	45.0	58.4	58.3	397-441 bp	1.0	80.9
atpI-rps2_p2	CTTGGGCATGGACTTGGAAA	TTGCAATTTGTGAGGGTCGT	50.0	45.0	58.4	58.3	398-442 bp	1.0	80.9
atpI-rps2_p3	ATGTTGGCCTACTTCCACAG	TGCAATTTGTGAGGGTCGTT	50.0	45.0	57.5	58.3	357-401 bp	1.0	80.1
atpI-rps2_p4	ATGTTGGCCTACTTCCACAG	TTGCAATTTGTGAGGGTCGT	50.0	45.0	57.5	58.3	358-402 bp	1.0	80.1
atpI-rps2_p5	AGTACTTGGGCATGGACTTG	TGCAATTTGTGAGGGTCGTT	50.0	45.0	57.5	58.3	401-445 bp	1.0	79.8