Hotspot

atpF-atpH

LSC · 13712-14482 · 771 bp

Back to Guibourtia

Pi 0.0967

MarkerSeek score 83.10

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 771
variable_sites: 121
pi: 0.0967
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	AAAGAAAGGAAAGAATGGGT	AGGCAGAGGGAAAAATACGG	35.0	50.0	52.5	57.6	752-997 bp	1.0	52.0
atpF-atpH_p2	AAGAAAGGAAAGAATGGGTT	AGGCAGAGGGAAAAATACGG	35.0	50.0	52.5	57.6	751-996 bp	1.0	52.0
atpF-atpH_p3	AAAGAAAGGAAAGAATGGGTT	AGGCAGAGGGAAAAATACGG	33.3	50.0	53.4	57.6	752-997 bp	1.0	52.0
atpF-atpH_p4	AAAGAAAGGAAAGAATGGGT	CCTGAGGCAGAGGGAAAAAT	35.0	50.0	52.5	57.5	756-1001 bp	1.0	52.0
atpF-atpH_p5	AAGAAAGGAAAGAATGGGTT	CCTGAGGCAGAGGGAAAAAT	35.0	50.0	52.5	57.5	755-1000 bp	1.0	52.0