Hotspot

psbI-trnS-GCU

LSC · 8133-8445 · 313 bp

Back to Guibourtia

Pi 0.0494

MarkerSeek score 76.90

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 313
variable_sites: 25
pi: 0.0494
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	TCCTATCTAACGATCCGGGAC	ATTTTCGTACCGAGGGTTCG	52.4	50.0	58.2	58.0	301-393 bp	1.0	80.2
psbI-trnS-GCU_p2	TCCTATCTAACGATCCGGGA	ATTTTCGTACCGAGGGTTCG	50.0	50.0	56.7	58.0	301-393 bp	1.0	79.8
psbI-trnS-GCU_p3	TCCTATCTAACGATCCGGGAC	TGGACTAAAGCGTCGGATTG	52.4	50.0	58.2	58.0	338-430 bp	1.0	79.6
psbI-trnS-GCU_p4	TCCTATCTAACGATCCGGGAC	GTGGACTAAAGCGTCGGATT	52.4	50.0	58.2	58.0	339-431 bp	1.0	79.6
psbI-trnS-GCU_p5	TCCTATCTAACGATCCGGGA	TGGACTAAAGCGTCGGATTG	50.0	50.0	56.7	58.0	338-430 bp	1.0	79.2