Hotspot

rbcL

LSC · 56561-58015 · 1455 bp

Back to Guadua

Pi 0
MarkerSeek score 34.50
Primer available yes
Label rbcL

Feature Metadata

label_name
rbcL
feature_type
gene
region
LSC
length_bp
1455
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
34.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL_p1 AGTAGACCCTGTCGTTGTGA AGCCGAGTTTAATTGCAATCA 50.0 38.1 58.0 56.5 1608 bp 1.0 60.0
rbcL_p2 ATCGAGTAGACCCTGTCGTT AGCCGAGTTTAATTGCAATCA 50.0 38.1 57.9 56.5 1612 bp 1.0 59.1
rbcL_p3 TAGACCCTGTCGTTGTGAGA AGCCGAGTTTAATTGCAATCA 50.0 38.1 57.7 56.5 1606 bp 1.0 57.8
rbcL_p4 AGTAGACCCTGTCGTTGTGA AGCCGAGTTTAATTGCAATCAA 50.0 36.4 58.0 57.2 1608 bp 1.0 57.7
rbcL_p5 GTAGACCCTGTCGTTGTGAG AGCCGAGTTTAATTGCAATCA 55.0 38.1 57.7 56.5 1607 bp 1.0 57.3