Hotspot

psbA

LSC · 88-1149 · 1062 bp

Back to Guadua

Pi 0.00502
MarkerSeek score 36.70
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
LSC
length_bp
1062
variable_sites
8
pi
0.00502
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
36.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 TCCTATATGTTAGTAGAAAAGCC AATACGTGTGCTTGGGAGTC 34.8 50.0 53.1 57.9 1135 bp 1.0 40.6
psbA_p2 TTCCTATATGTTAGTAGAAAAGCC AATACGTGTGCTTGGGAGTC 33.3 50.0 53.9 57.9 1136 bp 1.0 40.6
psbA_p3 TCCTATATGTTAGTAGAAAAGCC TGGGAGTCCTTGCAATTTGA 34.8 45.0 53.1 57.3 1123 bp 1.0 40.6
psbA_p4 TCCTATATGTTAGTAGAAAAGCC GCTTGGGAGTCCTTGCAATT 34.8 50.0 53.1 58.7 1126 bp 1.0 40.6
psbA_p5 ATTCCTATATGTTAGTAGAAAAGCC AATACGTGTGCTTGGGAGTC 32.0 50.0 54.5 57.9 1137 bp 1.0 40.6