Hotspot

ndhH

SSC · 114550-115731 · 1182 bp

Back to Guadua

Pi 0.00113
MarkerSeek score 59.50
Primer available yes
Label ndhH

Feature Metadata

label_name
ndhH
feature_type
gene
region
SSC
length_bp
1182
variable_sites
2
pi
0.00113
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH_p1 TCCCAGTAGGAGGGTCAAAA TGCATTTTGATGAGCCTCGT 50.0 45.0 57.6 58.2 1376 bp 1.0 75.3
ndhH_p2 TCCCAGTAGGAGGGTCAAAAT TGCATTTTGATGAGCCTCGT 47.6 45.0 58.1 58.2 1376 bp 1.0 70.2
ndhH_p3 TCCCAGTAGGAGGGTCAAAA TTGCATTTTGATGAGCCTCG 50.0 45.0 57.6 57.1 1377 bp 1.0 69.3
ndhH_p4 TCCCAGTAGGAGGGTCAAAA GCATTTTGATGAGCCTCGTT 50.0 45.0 57.6 57.1 1375 bp 1.0 69.3
ndhH_p5 TCCCAGTAGGAGGGTCAAAA TTTGCATTTTGATGAGCCTCG 50.0 42.9 57.6 57.8 1378 bp 1.0 66.9