Hotspot

ndhB

IRa · 128868-131112 · 2245 bp

Back to Guadua

Pi 0.00564
MarkerSeek score 48.30
Primer available yes
Label ndhB

Feature Metadata

label_name
ndhB
feature_type
gene
region
IRa
length_bp
2245
variable_sites
19
pi
0.00564
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhB_p1 ACAATCGGGACTTTTCGGAG CCATTTTGTTTTGGGCGGAA 50.0 45.0 57.9 57.8 2372 bp 1.0 77.9
ndhB_p2 ACAATCGGGACTTTTCGGAG TTGTTTTGGGCGGAACAGAT 50.0 45.0 57.9 58.3 2367 bp 1.0 77.5
ndhB_p3 ACAATCGGGACTTTTCGGAG TTTTGTTTTGGGCGGAACAG 50.0 45.0 57.9 57.7 2369 bp 1.0 77.5
ndhB_p4 ACAATCGGGACTTTTCGGAG AAGTTCATAACCCTAGCGCC 50.0 50.0 57.9 57.7 2397 bp 1.0 77.3
ndhB_p5 ACAATCGGGACTTTTCGGAG TTTGGGCGGAACAGATCTAC 50.0 50.0 57.9 57.6 2363 bp 1.0 76.7