Hotspot

atpF

LSC · 34816-36204 · 1389 bp

Back to Guadua

Pi 0.00096
MarkerSeek score 59.40
Primer available yes
Label atpF

Feature Metadata

label_name
atpF
feature_type
gene
region
LSC
length_bp
1389
variable_sites
2
pi
0.00096
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF_p1 GCGAGCGAAGTAAGTGATCA TTCGGAAGCAAGGGGAAAAA 50.0 45.0 58.1 57.9 1524-1530 bp 1.0 78.8
atpF_p2 GCGAGCGAAGTAAGTGATCA TTTCGGAAGCAAGGGGAAAA 50.0 45.0 58.1 57.9 1525-1531 bp 1.0 78.8
atpF_p3 GCGAGCGAAGTAAGTGATCA TTTTCGGAAGCAAGGGGAAA 50.0 45.0 58.1 57.9 1526-1532 bp 1.0 78.8
atpF_p4 GCGAGCGAAGTAAGTGATCA TTTTTCGGAAGCAAGGGGAA 50.0 45.0 58.1 57.9 1527-1533 bp 1.0 78.8
atpF_p5 AAAAAGGGCGAGCGAAGTAA TTCGGAAGCAAGGGGAAAAA 45.0 45.0 58.1 57.9 1531-1537 bp 1.0 78.6