Hotspot

rps15-ndhF

IRb · 102303-102747 · 445 bp

Back to Guadua

Pi 0.0187
MarkerSeek score 68.00
Primer available yes
Label rps15-ndhF

Feature Metadata

label_name
rps15-ndhF
feature_type
igs
region
IRb
length_bp
445
variable_sites
9
pi
0.0187
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ndhF_p1 TCGGAAAGAGGTCTACGAAGA TAGGGGGAGGACGAATATCG 47.6 55.0 57.9 57.8 639-685 bp 1.0 71.6
rps15-ndhF_p2 ACTTTTGGGAAAACGTCAACG TAGGGGGAGGACGAATATCG 42.9 55.0 57.9 57.8 619-665 bp 1.0 71.1
rps15-ndhF_p3 CGTTTGCTAGCTTATTTGGCA TAGGGGGAGGACGAATATCG 42.9 55.0 57.5 57.8 600-646 bp 1.0 68.4
rps15-ndhF_p4 ACGTTTGCTAGCTTATTTGGC TAGGGGGAGGACGAATATCG 42.9 55.0 57.5 57.8 601-647 bp 1.0 68.4
rps15-ndhF_p5 CGTCAACGTTTGCTAGCTTA TAGGGGGAGGACGAATATCG 45.0 55.0 56.5 57.8 606-652 bp 1.0 68.2