Hotspot

ndhC-trnV-UAC

LSC · 51963-52852 · 890 bp

Back to Guadua

Pi 0.00225
MarkerSeek score 60.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
890
variable_sites
3
pi
0.00225
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTAACCGGGGCTAAAAGTCC GGTTCGAATCCGTATAGCCC 55.0 55.0 58.0 57.9 1015-1017 bp 1.0 79.5
ndhC-trnV-UAC_p2 CTAACCGGGGCTAAAAGTCC CGGTTCGAATCCGTATAGCC 55.0 55.0 58.0 58.3 1016-1018 bp 1.0 77.7
ndhC-trnV-UAC_p3 TAACCGGGGCTAAAAGTCCT GGTTCGAATCCGTATAGCCC 50.0 55.0 58.3 57.9 1014-1016 bp 1.0 77.1
ndhC-trnV-UAC_p4 CACTAACCGGGGCTAAAAGT GGTTCGAATCCGTATAGCCC 50.0 55.0 57.5 57.9 1017-1019 bp 1.0 75.9
ndhC-trnV-UAC_p5 TAACCGGGGCTAAAAGTCCT CGGTTCGAATCCGTATAGCC 50.0 55.0 58.3 58.3 1015-1017 bp 1.0 75.2