Hotspot

atpI-atpH

LSC · 33292-34116 · 825 bp

Back to Guadua

Pi 0.00162
MarkerSeek score 59.90
Primer available yes
Label atpI-atpH

Feature Metadata

label_name
atpI-atpH
feature_type
igs
region
LSC
length_bp
825
variable_sites
2
pi
0.00162
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-atpH_p1 TAGCCGCAGCCTATATAGGT GCAGTACCTTGACCAACTCC 50.0 55.0 57.5 58.2 961 bp 1.0 74.5
atpI-atpH_p2 TAGCCGCAGCCTATATAGGT AAGCAGCAGCAATTAGTGGA 50.0 45.0 57.5 57.8 894 bp 1.0 74.4
atpI-atpH_p3 TAGCCGCAGCCTATATAGGT GCCAATCCAGCAGCAATAAC 50.0 50.0 57.5 57.8 916 bp 1.0 74.3
atpI-atpH_p4 TAGCCGCAGCCTATATAGGT ATAACGGAAGCAGCAGCAAT 50.0 45.0 57.5 58.2 901 bp 1.0 74.1
atpI-atpH_p5 ATTTTTGCAACGTTAGCCGC GCAGTACCTTGACCAACTCC 45.0 55.0 58.6 58.2 974 bp 1.0 74.0