Hotspot

psbM-petN

LSC · 18363-19143 · 781 bp

Back to Guadua

Pi 0
MarkerSeek score 61.80
Primer available yes
Label psbM-petN

Feature Metadata

label_name
psbM-petN
feature_type
igs
region
LSC
length_bp
781
variable_sites
0
pi
0
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-petN_p1 TGCTACTGCACTGTTCATTCT TAGTAAGTCTCGCTTGGGCT 42.9 50.0 57.6 58.2 937-943 bp 1.0 69.7
psbM-petN_p2 TGCTACTGCACTGTTCATTCT GTAGTATGGGGGAGGAGTGG 42.9 60.0 57.6 58.3 881-887 bp 1.0 68.8
psbM-petN_p3 TGCTACTGCACTGTTCATTCT TAGTATGGGGGAGGAGTGGA 42.9 55.0 57.6 58.4 880-886 bp 1.0 68.0
psbM-petN_p4 TGCTACTGCACTGTTCATTC TAGTAAGTCTCGCTTGGGCT 45.0 50.0 56.1 58.2 937-943 bp 1.0 65.5
psbM-petN_p5 TGCTACTGCACTGTTCATTCT AGTAGTATGGGGGAGGAGTG 42.9 55.0 57.6 57.2 882-888 bp 1.0 64.8