Hotspot

psbA-trnK-UUU

LSC · 1150-1385 · 236 bp

Back to Guadua

Pi 0.00282
MarkerSeek score 30.50
Primer available yes
Label psbA-trnK-UUU

Feature Metadata

label_name
psbA-trnK-UUU
feature_type
igs
region
LSC
length_bp
236
variable_sites
1
pi
0.00282
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
30.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnK-UUU_p1 GCTTGTACTTTCGCGTCTCT ACTCCATCCGACTAGTTCCG 50.0 55.0 58.3 58.6 314 bp 1.0 73.0
psbA-trnK-UUU_p2 CCCCACAGGCTTGTACTTTC ACTCCATCCGACTAGTTCCG 55.0 55.0 58.5 58.6 322 bp 1.0 71.7
psbA-trnK-UUU_p3 GCTTGTACTTTCGCGTCTCT ACTAGTTCCGGGTTCGAGTC 50.0 55.0 58.3 58.8 304 bp 1.0 71.2
psbA-trnK-UUU_p4 GCTTGTACTTTCGCGTCTCT GACTAGTTCCGGGTTCGAGT 50.0 55.0 58.3 58.8 305 bp 1.0 71.2
psbA-trnK-UUU_p5 GCTTGTACTTTCGCGTCTCT ATCCGACTAGTTCCGGGTTC 50.0 55.0 58.3 58.9 309 bp 1.0 70.7