Hotspot

ycf1

SSC · 124912-130554 · 5643 bp

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Pi 0.0163

MarkerSeek score 47.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5643
variable_sites: 92
pi: 0.0163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTGGCTTTTCTTGAGCGAA	AAAACAACGGACGAGTTCCA	45.0	45.0	57.7	58.0	1065-1071 bp	1.0	79.3
ycf1_p2	TCTGGCTTTTCTTGAGCGAA	GCAGAATACCGTCACCCATT	45.0	50.0	57.7	58.0	1304-1310 bp	1.0	79.0
ycf1_p3	ACACGTCTGGCTTTTCTTGA	AAAACAACGGACGAGTTCCA	45.0	45.0	57.7	58.0	1070-1076 bp	1.0	78.7
ycf1_p4	ACACGTCTGGCTTTTCTTGA	GCAGAATACCGTCACCCATT	45.0	50.0	57.7	58.0	1309-1315 bp	1.0	78.4
ycf1_p5	TCTGGCTTTTCTTGAGCGAA	AACGGACGAGTTCCACTTTC	45.0	50.0	57.7	58.1	1060-1066 bp	1.0	78.4