Hotspot

ccsA-ndhD

SSC · 116393-116661 · 269 bp

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Pi 0.0549

MarkerSeek score 73.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 269
variable_sites: 14
pi: 0.0549
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CAAATTCCGCAATTGTGGCT	CTCGACGAAATGGGTGGAAT	45.0	50.0	57.9	58.0	806-811 bp	1.0	82.1
ccsA-ndhD_p2	ATGCAGCTCTTTTATGCGGA	CTCGACGAAATGGGTGGAAT	45.0	50.0	58.0	58.0	1211-1216 bp	1.0	81.7
ccsA-ndhD_p3	TGCAGCTCTTTTATGCGGAT	CTCGACGAAATGGGTGGAAT	45.0	50.0	58.0	58.0	1210-1215 bp	1.0	81.7
ccsA-ndhD_p4	ACCCAAGGCTTTGCTACTTC	CTCGACGAAATGGGTGGAAT	50.0	50.0	58.1	58.0	1326-1331 bp	1.0	81.2
ccsA-ndhD_p5	CCCAAGGCTTTGCTACTTCA	CTCGACGAAATGGGTGGAAT	50.0	50.0	58.1	58.0	1325-1330 bp	1.0	81.2