Hotspot

rpl14-rpl16

LSC · 81997-82140 · 144 bp

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Pi 0.0493

MarkerSeek score 70.00

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 144
variable_sites: 7
pi: 0.0493
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	ACACATCAATTCTCGAGCCC	ACGAACTCAATTCATTATTTCGGG	50.0	37.5	58.0	57.9	234-235 bp	1.0	49.9
rpl14-rpl16_p2	AGCATATCGGCGATTGCTAG	ACGAACTCAATTCATTATTTCGGG	50.0	37.5	58.0	57.9	270-271 bp	1.0	49.7
rpl14-rpl16_p3	CATATCGGCGATTGCTAGCT	ACGAACTCAATTCATTATTTCGGG	50.0	37.5	58.0	57.9	268-269 bp	1.0	49.7
rpl14-rpl16_p4	GAGCATATCGGCGATTGCTA	ACGAACTCAATTCATTATTTCGGG	50.0	37.5	58.0	57.9	271-272 bp	1.0	49.7
rpl14-rpl16_p5	CATATCGGCGATTGCTAGCT	TGCCTATACGAACTCAATTCA	50.0	38.1	58.0	54.8	275-276 bp	1.0	48.7