Hotspot

psbH-petB

LSC · 75739-75859 · 121 bp

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Pi 0.0331

MarkerSeek score 71.50

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 121
variable_sites: 4
pi: 0.0331
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	GTCGCAATGGCTCTATTTGC	TGAAGATGACAGACCCCACT	50.0	50.0	57.9	58.0	276 bp	1.0	80.0
psbH-petB_p2	AACGACTCCTTTGATGGGTG	TGAAGATGACAGACCCCACT	50.0	50.0	57.8	58.0	295 bp	1.0	79.4
psbH-petB_p3	GGAACGACTCCTTTGATGGG	TGAAGATGACAGACCCCACT	55.0	50.0	58.3	58.0	297 bp	1.0	78.7
psbH-petB_p4	GGGAACGACTCCTTTGATGG	TGAAGATGACAGACCCCACT	55.0	50.0	58.3	58.0	298 bp	1.0	78.7
psbH-petB_p5	GGGGAACGACTCCTTTGATG	TGAAGATGACAGACCCCACT	55.0	50.0	58.3	58.0	299 bp	1.0	78.7