Hotspot

trnK-UUU-rps16

LSC · 4316-5117 · 802 bp

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Pi 0.0267
MarkerSeek score 70.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
802
variable_sites
21
pi
0.0267
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC AAAAAGGCGCTCAACCTACA 55.0 45.0 57.9 58.0 970-977 bp 1.0 82.1
trnK-UUU-rps16_p2 CGATGGTATGGACGAATCCC AAAAAGGCGCTCAACCTACA 55.0 45.0 57.9 58.0 969-976 bp 1.0 82.1
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 909-916 bp 1.0 81.6
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 908-915 bp 1.0 81.6
trnK-UUU-rps16_p5 TCAGTCGTGGTCTTCCAAAC AAAAAGGCGCTCAACCTACA 50.0 45.0 57.8 58.0 994-1001 bp 1.0 80.8