Pi
0.00793
MarkerSeek score
62.70
Primer available
yes
Label
clpP
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| clpP_p1 | TCGAAAATGAAATTCCAAATCCTGT | AGCTAATTCTTACGTTTCCACAC | 32.0 | 39.1 | 58.0 | 57.1 | 2146-2151 bp | 1.0 | 41.0 |
| clpP_p2 | TCGAAAATGAAATTCCAAATCCTGT | GCTAATTCTTACGTTTCCACACT | 32.0 | 39.1 | 58.0 | 57.1 | 2145-2150 bp | 1.0 | 41.0 |
| clpP_p3 | AGAGGATTCGAAAATGAAATTCCA | AGCTAATTCTTACGTTTCCACAC | 33.3 | 39.1 | 56.9 | 57.1 | 2153-2158 bp | 1.0 | 41.0 |
| clpP_p4 | AGAGGATTCGAAAATGAAATTCCA | GCTAATTCTTACGTTTCCACACT | 33.3 | 39.1 | 56.9 | 57.1 | 2152-2157 bp | 1.0 | 41.0 |
| clpP_p5 | CGAAAATGAAATTCCAAATCCTGT | GCTAATTCTTACGTTTCCACACT | 33.3 | 39.1 | 56.7 | 57.1 | 2144-2149 bp | 1.0 | 41.0 |